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PLoS One. 2017 Nov 30;12(11):e0187947. doi: 10.1371/journal.pone.0187947. eCollection 2017.

The infection and impact of Azorhizobium caulinodans ORS571 on wheat (Triticum aestivum L.).

Author information

1
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
2
College of Science, Northwest A&F University, Yangling, Shaanxi, China.
3
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
4
Department of Biology, East Carolina University, Greenville, NC, United States of America.

Abstract

Based on our previous study, cereal crop wheat (Triticum aestivum L.) could be infected by rhizobia Azorhizobium caulinodans ORS571, and form para-nodules with the induction of 2.4-dichlorophenoxyacetic acid, a common plant growth regulator. To enhance this infection and the potential agricultural application, we compared six different infection methods (Direct seed dip; Seed germination dip; Pruned-root dip; Foliar spray; Circum-soil dip; Seed dip and circum-soil dip) for achieving the high efficient infection of A. caulinodans into wheat plants by employing a green fluorescent protein (gfp)-labeled Azorhizobium caulinodans strain ORS571. With proper methods, copious rhizobia could enter the interior and promote the growth of wheat to the hilt. Circum-soil dip was proved to be the most efficient method, seed germination dip and pruned-root dip is the last recommended to infect wheat, seed germination dip and seed dip and circum-soil dip showed better effects on plant growth, pruned-root dip did not show too much effect on plant growth. This study laid the foundation for understanding the interaction between rhizobia and cereal crops and the growth-promoting function of rhizobia.

PMID:
29190702
PMCID:
PMC5708735
DOI:
10.1371/journal.pone.0187947
[Indexed for MEDLINE]
Free PMC Article

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