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BMC Res Notes. 2017 Nov 29;10(1):649. doi: 10.1186/s13104-017-2975-0.

D-β-Hydroxybutyrate and melatonin for treatment of porcine hemorrhagic shock and injury: a melatonin dose-ranging study.

Author information

1
Department of Surgery, University of Minnesota, 420 Delaware St SE, Minneapolis, MN, 55455, USA. awolf@umn.edu.
2
Department of Surgery, University of Minnesota, 420 Delaware St SE, Minneapolis, MN, 55455, USA.

Abstract

OBJECTIVE:

Treatment with a combination of D-β-hydroxybutyrate (BHB) and melatonin (M) improves survival in hemorrhagic shock models. Our objective was to find the most effective melatonin concentration in combination with 4 molar BHB (4 M BHB). Survival and markers of organ injury were analyzed in pigs exposed to pulmonary contusion, liver crush injury, and hemorrhagic shock and treated with lactated Ringer's solution; 4 M BHB/43 mM M; 4 M BHB/20 mM M; 4 M BHB/10 mM M; 4 M BHB/4.3 mM M; or 4 M BHB/0.43 mM M. This work is an extension of a previously published research study.

RESULTS:

Survival was highest in pigs receiving 4 M BHB/43 mM M (13/14), followed by lactated Ringer's solution (11/16) and BHB/M with decreased melatonin concentrations (4 M BHB/20 mM M 3/6, 4 M BHB/10 mM M 2/6, 4 M BHB/4.3 mM M 3/6, 4 M BHB/0.43 mM M 1/6, p = 0.011). High mortality was associated with increases in serum lactate, higher liver and muscle injury markers and decreases in PaO2:FiO2 ratios. Our study indicates that treatment with 4 M BHB and melatonin concentrations below 43 mM lack the survival benefit observed from 4 M BHB/43 mM melatonin in pigs experiencing hemorrhagic shock and polytrauma.

KEYWORDS:

Antioxidant; Blood loss; Hibernation; Ketone bodies; Melatonin; Resuscitation

PMID:
29187245
PMCID:
PMC5707828
DOI:
10.1186/s13104-017-2975-0
[Indexed for MEDLINE]
Free PMC Article

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