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Syst Biol. 2018 May 1;67(3):552-558. doi: 10.1093/sysbio/syx092.

Complex Models of Sequence Evolution Require Accurate Estimators as Exemplified with the Invariable Site Plus Gamma Model.

Author information

1
Center for Integrative Bioinformatics Vienna, Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Medical University Vienna, Campus Vienna Biocenter 5, A-1030, Vienna, Austria.
2
Bioinformatics and Computational Biology, Faculty of Computer Science, University of Vienna, Waehringer Strasse 29, A-1090 Vienna, Austria.

Abstract

The invariable site plus $\Gamma$ model (I$+\Gamma)$ is widely used to model rate heterogeneity among alignment sites in maximum likelihood and Bayesian phylogenetic analyses. The proof that the I$+$ continuous $\Gamma$ model is identifiable (model parameters can be inferred correctly given enough data) has increased the creditability of its application to phylogeny reconstruction. However, most phylogenetic software implement the I$+$ discrete $\Gamma$ model, whose identifiability is likely but unproven. How well the parameters of the I$+$ discrete $\Gamma$ model are estimated is still disputed. Especially the correlation between the fraction of invariable sites and the fractions of sites with a slow evolutionary rate is discussed as being problematic. We show that optimization heuristics as implemented in frequently used phylogenetic software (PhyML, RAxML, IQ-TREE, and MrBayes) cannot always reliably estimate the shape parameter, the proportion of invariable sites, and the tree length. Here, we propose an improved optimization heuristic that accurately estimates the three parameters. While research efforts mainly focus on tree search methods, our results signify the equal importance of verifying and developing effective estimation methods for complex models of sequence evolution.

PMID:
29186593
PMCID:
PMC6204645
DOI:
10.1093/sysbio/syx092
[Indexed for MEDLINE]
Free PMC Article

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