Format

Send to

Choose Destination
Nat Genet. 2017 Nov 29;49(12):1674-1675. doi: 10.1038/ng.3994.

Correcting CRISPR for copy number.

Author information

1
Department of Medicine, University of California, San Diego, La Jolla, California, USA.

Abstract

The CRISPR-Cas9 system enables global screens of gene function with high sensitivity and specificity, but off-target effects have been reported for CRISPR guide RNAs targeting genes that are amplified at high copy number. A new study describes a computational approach to correct for this copy number effect, increasing the specificity of CRIPSR screens to identify essential genes.

PMID:
29186130
DOI:
10.1038/ng.3994

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center