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Sci Rep. 2017 Nov 29;7(1):16575. doi: 10.1038/s41598-017-16857-6.

Metabolomics profiling reveals different patterns in an animal model of asphyxial and dysrhythmic cardiac arrest.

Author information

1
Medical School, National and Kapodistrian University of Athens, Athens, Greece.
2
European University Cyprus, Nicosia, Cyprus.
3
Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy.
4
Metabolic diseases Laboratory, Children Hospital "A. Cao", Cagliari, Italy.
5
Department of Surgical Sciences, University of Cagliari, Cagliari, Italy.
6
Aretaieio Hospital, National and Kapodistrian University of Athens, Athens, Greece.
7
Department of Chemical and Geological Sciences, University of Cagliari, Cagliari, Italy.
8
Institute for the Study of Macromolecules, ISMAC, National Council of Research, Lab, NMR, Milan, Italy.
9
Hellenic Society of Cardiopulmonary Resuscitation, Athens, Greece.
10
Experimental-Research Center ELPEN Pharmaceutical, Athens, Greece.
11
Public Health Institute, Catholic University of Rome, Rome, Italy.
12
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Caserta, Italy.
13
S-IN, Soluzioni Informatiche S.r.l., Vicenza, Italy.
14
Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy. elocci@unica.it.

Abstract

Cardiac arrest (CA) is not a uniform condition and its pathophysiology strongly depends on its cause. In this work we have used a metabolomics approach to study the dynamic metabolic changes occurring in the plasma samples of a swine model following two different causes of CA, namely asphyxia (ACA) and ventricular fibrillation (VFCA). Plasma samples were collected at baseline and every minute during the experimental phases. In order to identify the metabolomics profiles characterizing the two pathological entities, all samples were analysed by 1H NMR spectroscopy and LC-MS/MS spectrometry.The metabolomics fingerprints of ACA and VFCA significantly differed during the peri-arrest period and the resuscitation phase. Major alterations were observed in plasma concentrations of metabolites related to tricarboxylic acid (TCA) cycle, urea cycle, and anaplerotic replenishing of TCA. ACA animals showed significant metabolic disturbances during the asphyxial and CA phases, while for VFCA animals this phenomenon resulted shifted at the resuscitation phase. Interestingly, starting from the asphyxial phase, the ACA animals were stratified in two groups based on their metabolomics profiles that resulted to be correlated with the clinical outcome. Succinate overproduction was observed in the animals with the worse outcome, suggesting a potential prognostic role for this metabolite.

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