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Int J Mol Sci. 2017 Nov 28;18(12). pii: E2559. doi: 10.3390/ijms18122559.

PPARγ Modulates Long Chain Fatty Acid Processing in the Intestinal Epithelium.

Author information

1
Lee Kong Chian School of Medicine, Nanyang Technological University, 11 Mandalay Road, Singapore 308232, Singapore. Kalina.duszka@univie.ac.at.
2
Center for Integrative Genomics, University of Lausanne, Génopode, CH-1015 Lausanne, Switzerland. Kalina.duszka@univie.ac.at.
3
Department of Nutritional Sciences, University of Vienna, Althanstrasse 14, 1090 Vienna,Austria. Kalina.duszka@univie.ac.at.
4
Turku Centre for Biotechnology, University of Turku and Åbo Akademi University,Tykisokatu 6, 20520 Turku, Finland. matej.oresic@utu.fi.
5
Institut du Thorax, INSERM, CNRS, UNIV Nantes, 44007 Nantes, France. cedric.lemay@univ-nantes.fr.
6
Department of Nutritional Sciences, University of Vienna, Althanstrasse 14, 1090 Vienna,Austria. juergen.koenig@univie.ac.at.
7
Vienna Metabolomics Center (VIME), University of Vienna, Althanstrasse 14, 1090 Vienna, Austria. juergen.koenig@univie.ac.at.
8
Lee Kong Chian School of Medicine, Nanyang Technological University, 11 Mandalay Road, Singapore 308232, Singapore. Walter.Wahli@ntu.edu.sg.
9
Center for Integrative Genomics, University of Lausanne, Génopode, CH-1015 Lausanne, Switzerland. Walter.Wahli@ntu.edu.sg.
10
ToxAlim, Research Center in Food Toxicology, National Institute for Agricultural Research (INRA),180 Chemin de Tournefeuille, 31300 Toulouse, France. Walter.Wahli@ntu.edu.sg.

Abstract

Nuclear receptor PPARγ affects lipid metabolism in several tissues, but its role in intestinal lipid metabolism has not been explored. As alterations have been observed in the plasma lipid profile of ad libitum fed intestinal epithelium-specific PPARγ knockout mice (iePPARγKO), we submitted these mice to lipid gavage challenges. Within hours after gavage with long chain unsaturated fatty acid (FA)-rich canola oil, the iePPARγKO mice had higher plasma free FA levels and lower gastric inhibitory polypeptide levels than their wild-type (WT) littermates, and altered expression of incretin genes and lipid metabolism-associated genes in the intestinal epithelium. Gavage with the medium chain saturated FA-rich coconut oil did not result in differences between the two genotypes. Furthermore, the iePPARγKO mice did not exhibit defective lipid uptake and stomach emptying; however, their intestinal transit was more rapid than in WT mice. When fed a canola oil-rich diet for 4.5 months, iePPARγKO mice had higher body lean mass than the WT mice. We conclude that intestinal epithelium PPARγ is activated preferentially by long chain unsaturated FAs compared to medium chain saturated FAs. Furthermore, we hypothesize that the iePPARγKO phenotype originates from altered lipid metabolism and release in epithelial cells, as well as changes in intestinal motility.

KEYWORDS:

PPARγ; intestine; lipid metabolism

PMID:
29182565
PMCID:
PMC5751162
DOI:
10.3390/ijms18122559
[Indexed for MEDLINE]
Free PMC Article

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