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Exp Appl Acarol. 2017 Dec;73(3-4):493-499. doi: 10.1007/s10493-017-0189-8. Epub 2017 Nov 27.

Morphological and molecular divergence of Rhipicephalus turanicus tick from Albania and China.

Author information

1
School of Medicine, Shihezi University, Shihezi, 832002, Xinjiang Uygur Autonomous Region, People's Republic of China.
2
Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary.
3
College of Animal Science and Technology, Shihezi University, Shihezi, 832002, Xinjiang Uygur Autonomous Region, People's Republic of China.
4
Gene Cology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, QLD, 4556, Australia.
5
Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100029, People's Republic of China.
6
School of Medicine, Shihezi University, Shihezi, 832002, Xinjiang Uygur Autonomous Region, People's Republic of China. wangyuanzhi621@126.com.

Abstract

Rhipicephalus turanicus is an important tick species potentially carrying tick-borne pathogens. Several tick species have obvious subspecies divergence. However few studies aimed to examine the existence of divergence within R. turanicus. Therefore, a detailed morphological and molecular analysis was conducted for comparing R. turanicus from the Mediterranean Basin (represented by Albania) and Central Asia (Northwestern China). Altogether 315 adult ticks of R. turanicus (103 from Albania and 212 from China) were morphologically and molecularly analysed. DNA samples were used for mitochondrial 16S rRNA and cox1 gene sequences analysis. In addition, as potentially genetic markers, three fragments including partial nad1-16S rRNA, nad2-cox1, cox1-tRNA-Lys, were designed and then phylogenetically analyzed. Based on detailed morphological observations, only basis capituli length:width ratio (females), the length, the width and the length:width ratio of the scutum (males) had differences between R. turanicus from China and Albania. Gene divergences of 16S rRNA, cox1, partial nad1-16S rRNA, nad2-cox1 and cox1-tRNA-Lys from China and Albania ticks were 3.53-4.84, 3.57-4.92, 3.57-4.07, 3.57-4.39 and 3.18-4.69%, respectively. The evaluated five genetic markers revealed two phylogenetic branches in R. turanicus. Obvious differences exist within R. turanicus based on morphological and genetic analysis. Three newly designed genetic markers (partial nad1-16S rRNA, nad2-cox1 and cox1-tRNA-Lys) in this study may be suitable genetic tools for identification and analysis in R. turanicus. Subspecies analysis of R. turanicus from other regions of the world should be initiated in the future.

KEYWORDS:

Morphology; Phylogeny; Rhipicephalus turanicus; Subspecies

PMID:
29181674
PMCID:
PMC5727151
DOI:
10.1007/s10493-017-0189-8
[Indexed for MEDLINE]
Free PMC Article

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