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Biomed Rep. 2017 Nov;7(5):416-422. doi: 10.3892/br.2017.979. Epub 2017 Sep 11.

Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells.

Author information

1
Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan, R.O.C.
2
Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C.
3
University of Pennsylvania School of Dental Medicine, Philadelphia, PA 19104, USA.
4
Graduate Institute of Natural Products, School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C.
5
Department of Neurosurgery, E-Da Hospital, I-Shou University, Kaohsiung 84001, Taiwan, R.O.C.
6
Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C.

Abstract

Pluchea indica (L.) Less. is a perennial plant known for its versatile uses in traditional medicine. Previous findings have shown that the extracts of Pluchea indica possess significant anti-inflammatory, anti-ulcer and anti-tuberculosis activity. The aim of this study was to demonstrate the anticancer activity of the hexane fraction of P. indica root extract (H-PIRE) in human glioblastoma cells using flow cytometric and western blot analysis. The results shoewd that, H-PIRE suppressed the growth of glioblastoma cells in a dose-dependent manner. H-PIRE treatment markedly decreased the population of cells in S and G2/M phases. The significant upregulation of acidic vesicular organelle (AVO) was detected during H-PIRE treatment. The expression levels of microtubule-associated light chain 3-II (LC3-II) protein, phosphorylated JNK and phosphorylated p38 were significantly increased, confirming the occurrence of autophagy during the process. Finally, the combination treatment of H-PIRE and LY294002, a pan PI3K inhibitor, further decreased cell viability, suggesting an additive anticancer effect. Taken together, our results suggest that H-PIRE suppresses the proliferation of glioblastoma cells by inducing cell cycle arrest and autophagy.

KEYWORDS:

JNK; LC3-II; acidic vesicular organelle; anticancer; p38

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