Send to

Choose Destination
Chem Biodivers. 2018 Feb;15(2). doi: 10.1002/cbdv.201700507. Epub 2018 Feb 10.

Chemical Composition and In vitro Anthelmintic Activity of Extracts of Tagetes patula Against a Multidrug-Resistant Isolate of Haemonchus contortus.

Author information

Department of Organic Chemistry, Institute of Chemistry, UNESP - Univ Estadual Paulista, Rua Professor Francisco Degni 55, Bairro Quitandinha, CEP 14800-060, Araraquara, SP, Brazil.
Department of Drugs and Medicines, School of Pharmaceutical Sciences, UNESP - Univ Estadual Paulista, Rodovia Araraquara-Jaú Km 01 s/n, Bairro Campos Ville, CEP 14800-903, Araraquara, SP, Brazil.
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Pecuária Sudeste, Rodovia Washington Luiz Km 234 s/n, Fazenda Canchim, CEP 13560-970, São Carlos, SP, Brazil.
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Agroindústria de Alimentos, Avenida das Américas 29501, Bairro Guaratiba, CEP 23020-470, Rio de Janeiro, RJ, Brazil.


Sheep breeding has suffered economic losses due to parasitism by gastrointestinal nematodes, particularly Haemonchus contortus. The use of natural products, specifically Tagetes patula, has been suggested as an alternative method of combatting this issue. Chemical analyses of the extracts of this species described in the literature report the presence of important classes of secondary metabolites such as thiophenes, flavonoids, alkaloids, and benzofurans, some of which were identified and isolated in this study. The aim of this work was to test the effect of the essential oil (EO) and the ethanolic extract of the aerial parts (TpEtOH ) of T. patula on eggs and larvae of H. contortus, through an egg hatch test (EHT) and a larval development test (LDT). In the EHT, the EO showed 100% inhibition at 0.75 mg mL-1 (LC50 = 0.0780 mg mL-1 ), and the TpEtOH showed 100% inhibition at 100 mg mL-1 (LC50 = 12.8 mg mL-1 ). In the LDT, the EO showed 100% inhibition at 0.375 mg mL-1 (LC50 = 0.0400 mg mL-1 ), and the TpEtOH showed 100% inhibition at 1.56 mg mL-1 (LC50 = 0.340 mg mL-1 ). Compared to available literature data, the results presented here suggest that the crude extracts of T. patula have substantial potential for controlling this nematode by interrupting its life cycle and/or preventing it from reaching the infective stage.


Haemonchus contortus ; Tagetes patula ; natural products; terpenoids; thiophenes

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center