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Graefes Arch Clin Exp Ophthalmol. 2018 Feb;256(2):325-332. doi: 10.1007/s00417-017-3846-z. Epub 2017 Nov 25.

Plasma levels of hypoxia-regulated factors in patients with age-related macular degeneration.

Author information

1
Department of Ophthalmology, University Hospital Zurich, Frauenklinikstrasse 24, 8091, Zurich, Switzerland.
2
Lab for Retinal Cell Biology, Department of Ophthalmology, Zurich Centre for Integrative Human Physiology (ZIHP), and Neuroscience Centre (ZNZ), University of Zurich, Zurich, Switzerland.
3
Department of Ophthalmology, University Hospital Zurich, Frauenklinikstrasse 24, 8091, Zurich, Switzerland. dbar8165@uni.sydney.edu.au.

Abstract

PURPOSE:

Various hypoxia-related proteins are differentially expressed in the retina and secreted to the vitreous and/or aqueous humor of patients affected by dry or neovascular age-related macular degeneration (nAMD). To determine whether these conditions alter concentrations of cytokines also in the systemic circulation, we measured plasma levels of six hypoxia-related proteins.

METHODS:

Plasma was prepared from EDTA blood that was collected from patients affected by dry AMD (n = 5), nAMD (n = 11), proliferative diabetic retinopathy (PDR; n = 9), and patients with an epiretinal membrane (ERM; n = 11). ERM samples served as negative controls, PDR samples as positive controls. Protein concentrations of vascular endothelial growth factor (VEGF), erythropoietin (EPO), angiopoietin-like 4 (ANGPTL4), placental growth factor (PlGF), tumor necrosis factor alpha (TNF-α), and pigment epithelium-derived factor (PEDF) were determined by enzyme-linked immunosorbent assay (ELISA).

RESULTS:

The concentration of PlGF was significantly increased in plasma of patients affected by nAMD. Although no statistically significant differences were found for EPO, ANGPTL4, PlGF, TNF-α, and PEDF, the mean concentration of VEGF was lowest in the nAMD group. Plasma concentrations of the six factors did not correlate with gender or age of patients.

CONCLUSIONS:

nAMD may increase plasma concentrations of PlGF, making it a candidate as a biomarker for the neovascular form of AMD. Other factors, however, were not differentially regulated, suggesting that their systemic concentrations are not generally increased in hypoxia-related retinal diseases.

KEYWORDS:

Age-related macular degeneration; Cytokines; Plasma; Vascular endothelial growth factor

PMID:
29177891
PMCID:
PMC5790859
DOI:
10.1007/s00417-017-3846-z
[Indexed for MEDLINE]
Free PMC Article

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