Abstract
Heat shock proteins (HSP) are rapidly induced after stresses such as heat shock and accumulate at high concentrations in cells. HSP induction involves primarily a family of heat shock transcription factors (HSF) that bind the heat shock elements of the HSP genes and mediate transcription in trans. We discuss methods for the study of HSP binding to HSP promoters and the consequent increases in HSP gene expression in vitro and in vivo.
Keywords:
Binding; Chromatin immunoprecipitation; Heat shock factor; Heat shock protein; Nuclear run on; Purification; Transcription.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Chromatin Immunoprecipitation / methods*
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DNA / metabolism
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Electrophoretic Mobility Shift Assay / methods
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HeLa Cells
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Heat Shock Transcription Factors / metabolism
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Heat-Shock Proteins / isolation & purification
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Heat-Shock Proteins / metabolism*
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Heat-Shock Proteins / physiology
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Heat-Shock Response
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Humans
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Mice
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Molecular Biology / methods*
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NIH 3T3 Cells
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Promoter Regions, Genetic*
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Stress, Physiological*
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Transcription Factors / metabolism
Substances
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HSF1 protein, human
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Heat Shock Transcription Factors
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Heat-Shock Proteins
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Transcription Factors
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DNA