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Aquat Toxicol. 2018 Jan;194:112-120. doi: 10.1016/j.aquatox.2017.11.009. Epub 2017 Nov 21.

Metabolomics and transcriptomics reveal the toxicity of difenoconazole to the early life stages of zebrafish (Danio rerio).

Author information

1
College of Sciences, China Agricultural University, Beijing, China.
2
Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing, China.
3
Shandong Ruan Gas Company, Dongying, Shandong Province, China.
4
College of Sciences, China Agricultural University, Beijing, China. Electronic address: mqzheng@cau.edu.cn.
5
College of Sciences, China Agricultural University, Beijing, China. Electronic address: wangchengju@cau.edu.cn.

Abstract

Difenoconazole is widely used to inhibit the growth of fungi, but its residue in the water environment may threaten ecosystem and human health. Here, 1H nuclear magnetic resonance (NMR) and LC-MS/MS based metabolomics and transcriptomics approaches were used to assess the response of zebrafish to difenoconazole exposure. Early life stages of zebrafish were exposed to difenoconazole at environmentally relevant concentrations for 168h. Their comparison with the control group suggested an adverse development and disturbance of steroid hormones and VTG. KEGG pathway analysis identified five biological processes on the basis of differentially expressed genes (DEGs), as well as altered metabolites and amino acids in zebrafish following difenoconazole exposure. These affected processes included energy metabolism, amino acids metabolism, lipid metabolism, nucleotide metabolism, and an immune-related pathway. Collectively, these results bring us closer to an incremental understanding of the toxic effects of difenoconazole on zebrafish in its early development, and lend support to the continued use of the early life stages of zebrafish as a classical model to evaluate underlying environmental risks of xenobiotics in aquatic organisms.

KEYWORDS:

Difenoconazole; Metabolomics; Toxicity; Transcriptomics; Zebrafish

PMID:
29175743
DOI:
10.1016/j.aquatox.2017.11.009
[Indexed for MEDLINE]

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