Send to

Choose Destination
Anal Chem. 2017 Dec 19;89(24):13044-13048. doi: 10.1021/acs.analchem.7b04335. Epub 2017 Nov 29.

Endogenous Alpha-Synuclein Protein Analysis from Human Brain Tissues Using Single-Molecule Pull-Down Assay.

Author information

Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida 32827, United States.
CREOL, The College of Optics and Photonics, University of Central Florida , Orlando Florida 32816, United States.


Alpha-synuclein (α-SYN) is a central molecule in Parkinson's disease pathogenesis. Despite several studies, the molecular nature of endogenous α-SYN especially in human brain samples is still not well understood due to the lack of reliable methods and the limited amount of biospecimens. Here, we introduce α-SYN single-molecule pull-down (α-SYN SiMPull) assay combined with in vivo protein crosslinking to count individual α-SYN protein and assess its native oligomerization states from biological samples including human postmortem brains. This powerful single-molecule assay can be highly useful in diagnostic applications using various specimens for neurodegenerative diseases including Alzheimer's disease and Parkinson's disease.

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center