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Nat Immunol. 2018 Jan;19(1):85-97. doi: 10.1038/s41590-017-0001-2. Epub 2017 Nov 21.

Single-cell analysis reveals the continuum of human lympho-myeloid progenitor cells.

Author information

1
MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
2
Oxford Biomedical Research Centre, University of Oxford, Oxford, UK.
3
Department of Haematology and Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.
4
Division of Hematology, Stanford Institute for Stem Cell Biology and Regenerative Medicine, California, USA.
5
Department of Hematology, OUH NHS Trust, Oxford, UK.
6
UMR967 INSERM/CEA, Université Paris 7/Université Paris 11, Paris, France.
7
UMR1163, Imagine Institute, Paris Descartes -Sorbonne Paris Cité University, Paris, France.
8
MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK. paresh.vyas@imm.ox.ac.uk.
9
Oxford Biomedical Research Centre, University of Oxford, Oxford, UK. paresh.vyas@imm.ox.ac.uk.
10
Department of Hematology, OUH NHS Trust, Oxford, UK. paresh.vyas@imm.ox.ac.uk.

Abstract

The hierarchy of human hemopoietic progenitor cells that produce lymphoid and granulocytic-monocytic (myeloid) lineages is unclear. Multiple progenitor populations produce lymphoid and myeloid cells, but they remain incompletely characterized. Here we demonstrated that lympho-myeloid progenitor populations in cord blood - lymphoid-primed multi-potential progenitors (LMPPs), granulocyte-macrophage progenitors (GMPs) and multi-lymphoid progenitors (MLPs) - were functionally and transcriptionally distinct and heterogeneous at the clonal level, with progenitors of many different functional potentials present. Although most progenitors had the potential to develop into only one mature cell type ('uni-lineage potential'), bi- and rarer multi-lineage progenitors were present among LMPPs, GMPs and MLPs. Those findings, coupled with single-cell expression analyses, suggest that a continuum of progenitors execute lymphoid and myeloid differentiation, rather than only uni-lineage progenitors' being present downstream of stem cells.

PMID:
29167569
PMCID:
PMC5884424
DOI:
10.1038/s41590-017-0001-2
[Indexed for MEDLINE]
Free PMC Article

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