Format

Send to

Choose Destination
Am J Clin Pathol. 2017 Nov 20;148(6):502-512. doi: 10.1093/ajcp/aqx104.

Second Harmonic Generation Reveals Subtle Fibrosis Differences in Adult and Pediatric Nonalcoholic Fatty Liver Disease.

Author information

1
Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases, Beijing, China.
2
Department of Pathology and Hepatology, Beijing 302 Hospital, Beijing, China.
3
Institute of Molecular and Cell Biology, Singapore.
4
Department of Pathology, New York University School of Medicine, New York.
5
Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, National University Hospital, Singapore.

Abstract

Objectives:

Investigate subtle fibrosis similarities and differences in adult and pediatric nonalcoholic fatty liver disease (NAFLD) using second harmonic generation (SHG).

Methods:

SHG/two-photon excitation fluorescence imaging quantified 100 collagen parameters and determined qFibrosis values by using the nonalcoholic steatohepatitis (NASH) Clinical Research Network (CRN) scoring system in 62 adult and 36 pediatric NAFLD liver specimens.

Results:

Six distinct parameters identified differences among the NASH CRN stages with high accuracy (area under the curve, 0835-0.982 vs 0.885-0.981, adult and pediatric). All portal region parameters showed similar changes across early stages 0, 1C, and 2, in both groups. Parameter values decreased in adults with progression from stage 1A/B to 2 in the central vein region. In children, aggregated collagen parameters decreased, but nearly all distributed collagen parameters increased from stage 1A/B to 2.

Conclusions:

SHG analysis accurately reproduces NASH CRN staging in NAFLD, as well as reveals differences and similarities between adult and pediatric collagen deposition not captured by currently available quantitative methods.

KEYWORDS:

Adult; Automated quantitative analysis; Difference; Liver fibrosis; Nonalcoholic fatty liver disease; Pediatric; Second harmonic generation/two-photon excitation fluorescence

PMID:
29165568
DOI:
10.1093/ajcp/aqx104
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center