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Mar Drugs. 2017 Nov 22;15(11). pii: E364. doi: 10.3390/md15110364.

High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers.

Yi Y1,2, You X3, Bian C4, Chen S5,6, Lv Z7, Qiu L8, Shi Q9,10,11.

Author information

1
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China. yiyunhai@genomics.cn.
2
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China. yiyunhai@genomics.cn.
3
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China. youxinxin@genomics.cn.
4
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China. bianchao@genomics.cn.
5
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China. chenshixi@xmu.edu.cn.
6
Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen University, Xiamen 361102, China. chenshixi@xmu.edu.cn.
7
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. 18012481396@163.com.
8
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. qiulimei@qdio.ac.cn.
9
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China. shiqiong@genomics.cn.
10
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China. shiqiong@genomics.cn.
11
Laboratory of Aquatic Genomics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. shiqiong@genomics.cn.

Abstract

Widespread existence of antimicrobial peptides (AMPs) has been reported in various animals with comprehensive biological activities, which is consistent with the important roles of AMPs as the first line of host defense system. However, no big-data-based analysis on AMPs from any fish species is available. In this study, we identified 507 AMP transcripts on the basis of our previously reported genomes and transcriptomes of two representative amphibious mudskippers, Boleophthalmus pectinirostris (BP) and Periophthalmus magnuspinnatus (PM). The former is predominantly aquatic with less time out of water, while the latter is primarily terrestrial with extended periods of time on land. Within these identified AMPs, 449 sequences are novel; 15 were reported in BP previously; 48 are identically overlapped between BP and PM; 94 were validated by mass spectrometry. Moreover, most AMPs presented differential tissue transcription patterns in the two mudskippers. Interestingly, we discovered two AMPs, hemoglobin β1 and amylin, with high inhibitions on Micrococcus luteus. In conclusion, our high-throughput screening strategy based on genomic and transcriptomic data opens an efficient pathway to discover new antimicrobial peptides for ongoing development of marine drugs.

KEYWORDS:

amphibious mudskipper; antimicrobial activity; antimicrobial peptide (AMP); high-throughput identification

PMID:
29165344
PMCID:
PMC5706053
DOI:
10.3390/md15110364
[Indexed for MEDLINE]
Free PMC Article

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