Systemic and Local Cytokine Profile following Spinal Cord Injury in Rats: A Multiplex Analysis

Yana O Mukhamedshina; Elvira R Akhmetzyanova; Ekaterina V Martynova; Svetlana F Khaiboullina; Luisa R Galieva; Albert A Rizvanov

doi:10.3389/fneur.2017.00581

Systemic and Local Cytokine Profile following Spinal Cord Injury in Rats: A Multiplex Analysis

Front Neurol. 2017 Oct 31:8:581. doi: 10.3389/fneur.2017.00581. eCollection 2017.

Authors

Yana O Mukhamedshina^{1

2}, Elvira R Akhmetzyanova¹, Ekaterina V Martynova¹, Svetlana F Khaiboullina^{1

3}, Luisa R Galieva¹, Albert A Rizvanov¹

Affiliations

¹ Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.
² Kazan State Medical University, Kazan, Russia.
³ University of Nevada, Reno, NV, United States.

Abstract

Our study of the changes in cytokine profile in blood serum and in the spinal cord after traumatic spinal cord injury (SCI) has shown that an inflammatory reaction and immunological response are not limited to the CNS, but widespread. This fact was confirmed by changes detected in a cytokine profile in blood serum samples [MIP-1α, interleukin 1 (IL-1) α, IL-2, IL-5, IL-1β, MCP-1, RANTES]. There were also changes in the levels of MIP-1α, IL-1α, IL-2, IL-5, IL-18, GM-colony-stimulating factor, IL-17α, IFN-γ, IL-10, IL-13, MCP-1, and GRO KC CINC-1 in samples of the rat injured spinal cord. The results underscore the complex cytokine network imbalance exhibited after SCI and show significant changes in the concentrations of 14 cytokines/chemokines with different inflammatory and immunological activities.

Keywords: acute and subacute periods; cytokine profile; multiplex analysis; rats; spinal cord injury.