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Biochem Biophys Res Commun. 2018 Jan 1;495(1):1048-1054. doi: 10.1016/j.bbrc.2017.11.108. Epub 2017 Nov 21.

Visualization of local phosphatidylcholine synthesis within hippocampal neurons using a compartmentalized culture system and imaging mass spectrometry.

Author information

1
Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan; International Mass Imaging Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan; Department of Biochemistry, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo, Japan. Electronic address: esugiyama@keio.jp.
2
International Mass Imaging Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan; Department of Optical Imaging, Preeminent Medical Photonics Education & Research Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan. Electronic address: yaoik@hama-med.ac.jp.
3
Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan; International Mass Imaging Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan; Department of Systems Molecular Anatomy, Preeminent Medical Photonics Education & Research Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan; Department of Anatomy, The University of Hong Kong, 6/F, William MW Mong Block 21 Sassoon Road, Pokfulam, Hong Kong SAR, China. Electronic address: setou@hama-med.ac.jp.

Abstract

Neurons extend neurites with an increased synthesis of phosphatidylcholine (PC) that is not only a membrane component but also a functional regulator with specific fatty acid composition. To analyze the local synthesis of the PC molecular species within neurons, we combined a compartmentalized culture system with matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). We observed that a newly synthesized PC, which contains exogenously administered palmitic acid-d3, is accumulated at the cell bodies and the tips of the distal neurites. The local accumulation within distal neurites is formed by distinct metabolic activity from cell bodies, suggesting that the local extracellular composition of free fatty acid can be a key to regulate specific functions of each PC molecular species. We expect our simple method to be a starting point for more sophisticated in vitro analytical methods for unveiling detailed lipid metabolisms within neurons.

KEYWORDS:

Compartmentalized culture; Fatty acid; Imaging mass spectrometry; Microfluidic device; Neuron; Phosphatidylcholine

PMID:
29162450
DOI:
10.1016/j.bbrc.2017.11.108
[Indexed for MEDLINE]

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