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Nat Commun. 2017 Nov 20;8(1):1627. doi: 10.1038/s41467-017-01560-x.

Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling.

Author information

1
ACRF Stem Cells and Cancer Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.
2
Department of Medical Biology, The University of Melbourne, Parkville, VIC, 3010, Australia.
3
Bioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.
4
Australian Genome Research Facility, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.
5
Systems Biology & Personalised Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.
6
Department of Biochemistry and Molecular Biology, The University of Melbourne, Parkville, VIC, 3010, Australia.
7
Department of Medicine, The University of Melbourne, Parkville, VIC, 3010, Australia.
8
Parkville Familial Cancer Centre and Department of Medical Oncology, The Royal Melbourne Hospital and Peter MacCallum Cancer Centre, Parkville, VIC, 3050, Australia.
9
School of Mathematics and Statistics, The University of Melbourne, Parkville, VIC, 3010, Australia.
10
ACRF Stem Cells and Cancer Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia. visvader@wehi.edu.au.
11
Department of Medical Biology, The University of Melbourne, Parkville, VIC, 3010, Australia. visvader@wehi.edu.au.

Abstract

The mammary epithelium comprises two primary cellular lineages, but the degree of heterogeneity within these compartments and their lineage relationships during development remain an open question. Here we report single-cell RNA profiling of mouse mammary epithelial cells spanning four developmental stages in the post-natal gland. Notably, the epithelium undergoes a large-scale shift in gene expression from a relatively homogeneous basal-like program in pre-puberty to distinct lineage-restricted programs in puberty. Interrogation of single-cell transcriptomes reveals different levels of diversity within the luminal and basal compartments, and identifies an early progenitor subset marked by CD55. Moreover, we uncover a luminal transit population and a rare mixed-lineage cluster amongst basal cells in the adult mammary gland. Together these findings point to a developmental hierarchy in which a basal-like gene expression program prevails in the early post-natal gland prior to the specification of distinct lineage signatures, and the presence of cellular intermediates that may serve as transit or lineage-primed cells.

PMID:
29158510
PMCID:
PMC5696379
DOI:
10.1038/s41467-017-01560-x
[Indexed for MEDLINE]
Free PMC Article

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