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Prostaglandins Leukot Essent Fatty Acids. 2017 Dec;127:20-24. doi: 10.1016/j.plefa.2017.10.004. Epub 2017 Oct 7.

Coconut phytocompounds inhibits polyol pathway enzymes: Implication in prevention of microvascular diabetic complications.

Author information

1
Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, KAU P.O, Vellanikkara, Kerala 680656, India; Department of Biochemistry, Amala Cancer Research Centre (Recognized Research Centre of University of Calicut), Amala Nagar P.O., Thrissur, Kerala 680555, India. Electronic address: devilekshmys@gmail.com.
2
Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, KAU P.O, Vellanikkara, Kerala 680656, India; Department of Biochemistry, Amala Cancer Research Centre (Recognized Research Centre of University of Calicut), Amala Nagar P.O., Thrissur, Kerala 680555, India.

Abstract

Coconut oil (CO), the primary choice of cooking purposes in the south Asian countries, is rich in medium chain saturated fatty acids, especially lauric acid (50-52%). The oil has high medicinal use in Ayurvedic system and known to contain polyphenolic antioxidants. Studies have reported that CO improves insulin sensitivity and shows hypoglycemic effect. However, there is no information regarding its effect on chronic diabetic complications including retinopathy and nephropathy is available. The secondary diabetic complications are mediated by the activation of polyol pathway, where aldose reductase (AR) plays crucial role. In this study, in silico analysis has been used to screen the effect of CO as well as its constituents, MCFAs and phenolic compounds, for targeting the molecules in polyol pathway. The study revealed that lauric acid (LA) interacts with AR and DPP-IV of polyol pathway and inhibits the activity of these enzymes. Validation studies using animal models confirmed the inhibition of AR and SDH in wistar rats. Further, the LA dose dependently reduced the expression of AR in HCT-15 cells. Together, the study suggests the possible role of CO, particularly LA in reducing secondary diabetic complications.

KEYWORDS:

Aldose reductase; Coconut oil; Diabetes; Dipeptidyl peptidase-4; Lauric acid; Medium chain saturated fatty acid

PMID:
29156154
DOI:
10.1016/j.plefa.2017.10.004
[Indexed for MEDLINE]

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