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J Vis Exp. 2017 Oct 18;(128). doi: 10.3791/56282.

Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition.

Author information

1
Department of Neuroscience, University of Florida; McKnight Brain Institute, University of Florida; jflint@mbi.ufl.edu.
2
McKnight Brain Institute, University of Florida; Department of Biomedical Engineering, University of Florida.
3
Center for Functionally Integrative Neuroscience, Aarhus University.
4
McKnight Brain Institute, University of Florida; Department of Biomedical Engineering, University of Florida; Department of Radiology, University of Florida.
5
Department of Neuroscience, University of Florida; McKnight Brain Institute, University of Florida; National High Magnetic Field Laboratory, Florida State University.

Abstract

This protocol describes the procedures necessary to support normal metabolic functions of acute brain slice preparations during the collection of magnetic resonance (MR) microscopy data. While it is possible to perform MR collections on living, excised mammalian tissue, such experiments have traditionally been constrained by resolution limits and are thus incapable of visualizing tissue microstructure. Conversely, MR protocols that did achieve microscopic image resolution required the use of fixed samples to accommodate the need for static, unchanging conditions over lengthy scan times. The current protocol describes the first available MR technique that enables imaging of living, mammalian tissue samples at microscopic resolutions. Such data is of great importance to the understanding of how pathology-based contrast changes occurring at the microscopic level influence the content of macroscopic MR scans such as those used in the clinic. Once such an understanding is realized, diagnostic methods with greater sensitivity and accuracy can be developed, which will translate directly to earlier disease treatment, more accurate therapy monitoring and improved patient outcomes. While the described methodology focuses on brain slice preparations, the protocol is adaptable to any excised tissue slice given that changes are made to the gas and perfusate preparations to accommodate the tissue's specific metabolic needs. Successful execution of the protocol should result in living, acute slice preparations that exhibit MR diffusion signal stability for periods up to 15.5 h. The primary advantages of the current system over other MR compatible perfusion apparatuses are its compatibility with the MR microscopy hardware required to attain higher resolution images and ability to provide constant, uninterrupted flow with carefully regulated perfusate conditions. Reduced sample throughput is a consideration with this design as only one tissue slice may be imaged at a time.

PMID:
29155793
PMCID:
PMC5752427
DOI:
10.3791/56282
[Indexed for MEDLINE]
Free PMC Article

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