Format

Send to

Choose Destination
J Allergy Clin Immunol. 2018 Jul;142(1):246-257. doi: 10.1016/j.jaci.2017.10.030. Epub 2017 Nov 16.

Type I IFN-related NETosis in ataxia telangiectasia and Artemis deficiency.

Author information

1
Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
2
Department of Immunology and Allergy, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey.
3
Thorlab, Therapeutic Oligodeoxynucleotide Research Laboratory, Department of Molecular Biology and Genetics, Ihsan Dogramaci Bilkent University, Ankara, Turkey.
4
Department of Pathology, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey.
5
Department of Molecular Biology and Genetics, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
6
Department of Radiology, Hacettepe University Medical Faculty, Ankara, Turkey.
7
Department of Pediatric Immunology, Hacettepe University Medical Faculty, Ankara, Turkey.
8
Department of Pediatric Rheumatology, Hacettepe University Medical Faculty, Ankara, Turkey.
9
Department of Biological Sciences, Middle East Technical University, Ankara, Turkey. Electronic address: mgursel@metu.edu.tr.

Abstract

BACKGROUND:

Pathological inflammatory syndromes of unknown etiology are commonly observed in ataxia telangiectasia (AT) and Artemis deficiency. Similar inflammatory manifestations also exist in patients with STING-associated vasculopathy in infancy (SAVI).

OBJECTIVE:

We sought to test the hypothesis that the inflammation-associated manifestations observed in patients with AT and Artemis deficiency stem from increased type I IFN signature leading to neutrophil-mediated pathological damage.

METHODS:

Cytokine/protein signatures were determined by ELISA, cytometric bead array, or quantitative PCR. Stat1 phosphorylation levels were determined by flow cytometry. DNA species accumulating in the cytosol of patients' cells were quantified microscopically and flow cytometrically. Propensity of isolated polymorhonuclear granulocytes to form neutrophil extracellular traps (NETs) was determined using fluorescence microscopy and picogreen assay. Neutrophil reactive oxygen species levels and mitochondrial stress were assayed using fluorogenic probes, microscopy, and flow cytometry.

RESULTS:

Type I and III IFN signatures were elevated in plasma and peripheral blood cells of patients with AT, Artemis deficiency, and SAVI. Chronic IFN production stemmed from the accumulation of DNA in the cytoplasm of AT and Artemis-deficient cells. Neutrophils isolated from patients spontaneously produced NETs and displayed indicators of oxidative and mitochondrial stress, supportive of their NETotic tendencies. A similar phenomenon was also observed in neutrophils from healthy controls exposed to patient plasma samples or exogeneous IFN-α.

CONCLUSIONS:

Type I IFN-mediated neutrophil activation and NET formation may contribute to inflammatory manifestations observed in patients with AT, Artemis deficiency, and SAVI. Thus, neutrophils represent a promising target to manage inflammatory syndromes in diseases with active type I IFN signature.

KEYWORDS:

Artemis deficiency; NETosis; Primary immunodeficiencies; ataxia telangiectasia; autoinflammation; interferonopathy; neutrophil extracellular traps; type I IFN

PMID:
29155101
DOI:
10.1016/j.jaci.2017.10.030

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center