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Pregnancy Hypertens. 2017 Oct;10:207-212. doi: 10.1016/j.preghy.2017.09.002. Epub 2017 Sep 11.

Various levels of circulating exosomal total-miRNA and miR-210 hypoxamiR in different forms of pregnancy hypertension.

Author information

1
1st Dept. of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary. Electronic address: biro.orsolya@noi1.sote.hu.
2
1st Dept. of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary.
3
Dept. of Human Genetics, University of Debrecen, Hungary.

Abstract

INTRODUCTION:

Hypertension is a common complication during pregnancy, affecting 10% of pregnant women worldwide. Several microRNA (miRNA) were shown to be involved in hypertensive disorders of pregnancy. In preeclampsia (PE), placental dysfunction causes the enhanced release of extracellular vesicle-derived miRNAs. The hypoxia-sensitive hsa-mir-210 is the most common PE-associated miRNA, but its exosomal profile has not been investigated.

OBJECTIVES:

Our aims were to measure exosomal total-miRNA concentration and to perform expression analysis of circulating exosomal hsa-miR-210 in women affected by chronic hypertension (CHT) gestational hypertension (GHT) or PE.

MATERIALS AND METHODS:

We collected plasma samples from women with CHT, GHT, PE (moderate: mPE and severe: sPE) and from normotensive pregnancies. Exosomal miRNAs were extracted and miRNA concentration was measured. RT-PCR was carried out with hsa-miR-210-3p-specific primers and relative expression was calculated using the comparative Ct method.

RESULTS:

The total-miRNA concentration was different in the disease subgroups, and was significantly higher in mPE and sPE compared to the other groups. We found a significant difference in the relative exosomal hsa-miR-210-3p expression between all hypertensive groups compared to the normotensive samples, but significant upregulation was only observed in case of mPE and sPE patients. Both the level of total-miRNA and hsa-miR-210 expression was higher in case of severe PE.

CONCLUSIONS:

The level of circulating exosomal total-miRNA and hsa-miR-210 was elevated in women with PE, and it was higher in the severe form. We showed that hsa-miR-210 is secreted via exosomes, which may have a role in the pathomechanism of the disease.

KEYWORDS:

Exosome; Maternal circulation; Preeclampsia; microRNA

PMID:
29153681
DOI:
10.1016/j.preghy.2017.09.002
[Indexed for MEDLINE]

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