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J Cosmet Dermatol. 2018 Dec;17(6):1216-1224. doi: 10.1111/jocd.12453. Epub 2017 Nov 19.

Cosmeceutical effect of ethyl acetate fraction of Kombucha tea by intradermal administration in the skin of aged mice.

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Division of Immunology, Medical School, Alborz University of Medical Sciences, Karaj, Iran.
Department of Parasitology and Mycology, Medical School, Alborz University of Medical Sciences, Karaj, Iran.
Department of Pathology, Medical School, Alborz University of Medical Sciences, Karaj, Iran.
Department of Operating Room Nursing, Paramedical School, Alborz University of Medical Sciences, Karaj, Iran.
Herbal Medicine Research Center, Institute of Medicinal Plant, ACECR, Karaj, Iran.
Health, Safety, and Environment Research Center, Alborz University of Medical Sciences, Karaj, Iran.
Department of Food Science and Technology, Faculty of Agriculture, Islamic Azad University, Shahrekord, Iran.



Natural ingredients have been always an interesting approach to prolong youthful appearance of skin. One of the natural compounds is Kombucha tea (KT), which has been mainly used as an energy drink in Asian countries for a long time. Previous reports indicated that it has pharmaceutical and favorable wound repairing effects. The beneficial properties of KT are thought to be mainly due to the presence of fermentation products such as flavonoids and other polyphenols with inhibition of hydrolytic and oxidative enzymes and anti-inflammatory effects. These properties prompted us to study the anti-aging potential of KT and investigate its effective fraction in aged mice, METHODS: Kombucha tea was fractionated into chloroform, butanol, and ethyl acetate, and flavonoid content was determined. Young and old mice were used as control. KT ethyl acetate fraction (KEAf), which had the highest flavonoid content, was intradermally administered to old mice.


Administration of KEAf significantly increased the collagen content, NAD+ /NADH level, and concomitantly improved skin connective tissue abnormalities in the aged skin. No sensitivity or irritation was observed.


This finding suggested that KEAf can be a suitable candidate as a cosmetic product to improve aging-related skin abnormalities and regeneration of aged skin.


Kombucha tea; NAD+/NADH; aging; collagen; ethyl acetate fraction


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