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BMJ Open. 2017 Nov 16;7(11):e014549. doi: 10.1136/bmjopen-2016-014549.

Comprehensive analysis of vitreous specimens for uveitis classification: a prospective multicentre observational study.

Author information

1
Department of Ophthalmology and Visual Science, Tohoku University Graduate School of Medicine, Sendai, Japan.
2
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
3
Infection Control and Laboratory Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan.
4
RIKEN Center for Development Biology, Kobe, Japan.
5
Department of Hematopathology, Tohoku University Graduate School of Medicine, Sendai, Japan.
6
Department of Ophthalmology, Tokyo Medical Dental University, Tokyo, Japan.

Abstract

PURPOSE:

To determine the clinical relevance of vitreous biomarkers in patients with uveitis.

DESIGN:

Multicentre, prospective, observational study.

SETTING:

Uveitis outpatient clinics of two academic medical centres in Japan.

PATIENT POPULATION:

This study included 234 eyes of 191 patients with various uveitis aetiologies: definitive sarcoidosis (61 eyes of 46 patients), suspected sarcoidosis (60 eyes of 45 patients), intraocular tumour (34 eyes of 27 patients), viral infection (20 eyes of 18 patients), non-sarcoidosis (16 eyes of 16 patients) and unknown aetiology (43 eyes of 39 patients).

OBSERVATION PROCEDURE:

Vitreous samples (taken by pars planta vitrectomy) were analysed with flow cytometry, cytology and multiplex PCR analysis.

MAIN OUTCOME MEASURES:

The primary outcome measures were the diagnostic values of various biomarkers (T cells, B cells and pathogen DNA) in vitreous samples. The secondary outcome was visual acuity after vitrectomy.

RESULTS:

Sarcoidosis showed higher CD4/CD8 or CD4+ measurements than other aetiologies (p<0.01). In samples with viral infection, pathogen DNA was detected, and CD8+ counts were higher than the other aetiologies (p<0.01). Eyes with tumour had higher CD19+ (p<0.05). Non-sarcoidosis had lower CD4/CD8 than sarcoidosis, higher CD8+ than sarcoidosis and lower CD19+ than tumour (p<0.01). Unknown uveitis had lower CD4/CD8 than sarcoidosis (p<0.01), and higher CD4/CD8 than non-sarcoidosis, viral infection or tumour (p<0.001). Visual acuity improved after vitrectomy (p<0.001).

CONCLUSIONS:

Uveitis aetiologies had distinct vitreous biomarker profiles, especially of infiltrating lymphocytes. Analyses of CD4/CD8 ratio, T-lymphocyte and B-lymphocyte subset, and pathogen DNA in vitreous samples have good safety profiles and high diagnostic value for uveitis classification.

TRIAL REGISTRATION NUMBER:

UMIN000004980; Pre-results.

KEYWORDS:

cd4/cd8 ratio; flowcytometry analysis; uveitis; vitreous sample

PMID:
29150462
PMCID:
PMC5701993
DOI:
10.1136/bmjopen-2016-014549
[Indexed for MEDLINE]
Free PMC Article

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