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Transplant Proc. 2017 Nov;49(9):2031-2035. doi: 10.1016/j.transproceed.2017.09.040.

Comparison Between Total IgG, C1q, and C3d Single Antigen Bead Assays in Detecting Class I Complement-Binding Anti-HLA Antibodies.

Author information

1
Servicio de Cirugía General, Clínica Anglo Americana, Lima, Perú.
2
Division of Nephrology, Mississippi Baptist Medical Center, Jackson, Mississippi, USA.
3
Division of Health Sciences Research, Mayo Clinic, Rochester, Minnesota, USA.
4
Division of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, USA.
5
Division of Transplantation Surgery, Mayo Clinic, Rochester, Minnesota, USA.
6
Division of Transfusion Medicine, Mayo Clinic, Rochester, Minnesota, USA.
7
Division of Transfusion Medicine, Mayo Clinic, Rochester, Minnesota, USA. Electronic address: Gandhi.Manish@mayo.edu.

Abstract

BACKGROUND:

Complement-binding donor-specific antibodies (DSAs) are associated with antibody-mediated rejection and allograft loss. Novel single antigen bead (SAB) assays-that is, complement component 1q (C1q) and complement component 3d (C3d) assays-have been developed to specifically detect complement-binding DSA, but it remains unclear whether these assays have an improved ability to detect complement-binding DSA as compared with using the total IgG SAB assay with a high mean fluorescence intensity (MFI) cutoff. The aim of this study was to compare the ability of the total IgG, C1q, and C3d SAB assays in detecting complement-binding anti-HLA antibodies.

METHODS:

Twenty sera known to have complement-binding anti-HLA antibodies (serologic class I HLA typing by complement-dependent cytotoxicity method) were tested with 3 different SAB assays: total IgG (undiluted and 1:8 dilution), C1q, and C3d. Serologic anti-HLA specificities were compared with those obtained by IgG, C1q, and C3d SAB assays.

RESULTS:

IgG SAB was more sensitive in detecting complement-binding antibodies (sensitivity 24 of 24 = 1, odds ratio infinity). Pearson correlation showed the association between (1) C1q and IgG SAB assays (cutoff C1q SAB 1000 MFI, cutoff IgG SAB 5000 MFI: r = 0.347, P < .0001) and (2) C3d and IgG SAB assays (cutoff 500 MFI C3d SAB, 5000 MFI for IgG SAB: r = -0.173, P = .279).

CONCLUSIONS:

For class I anti-HLA antibodies, IgG SAB (cutoff MFI > 5000) was more sensitive in detecting complement-binding antibodies when compared with C1q and C3d SAB assays.

[Indexed for MEDLINE]

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