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Biomed Chromatogr. 2018 Apr;32(4). doi: 10.1002/bmc.4143. Epub 2017 Dec 20.

Determination of cefoperazone and sulbactam in serum by HPLC-MS/MS: An adapted method for therapeutic drug monitoring in children.

Author information

1
Laboratory of Clinical Pharmacokinetic of TCM, Affiliated Hospital of Liaoning University of Traditional Medicine, Shenyang, China.
2
Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.
3
Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Shandong University, Jinan, China.

Abstract

A rapid, accurate and specific high-performance liquid chromatography-tandem mass spectrometry method has been validated for the simultaneous determination of cefoperazone and sulbactam in a small volume sample for children. A Shim-pack XR-ODS C18 column with gradient elution of water (0.1% formic acid) and acetonitrile (0.1% formic acid) solution was used for separation at a flow rate of 0.3 mL/min. The calibration curves of two analytes in serum showed excellent linearity over the concentration ranges of 0.03-10 μg/mL for cefoperazone, and 0.01-3 μg/mL for sulbactam, respectively. This method involves simple sample preparation steps and was validated according to standard US Food and Drug Administration and European Medicines Agency guidelines in terms of selectivity, linearity, detection limits, matrix effects, accuracy, precision, recovery and stability. This assay can be easily implemented in clinical practice to determine concentrations of cefoperazone and sulbactam in children.

KEYWORDS:

HPLC-MS/MS; cefoperazone; children; sulbactam

PMID:
29148595
DOI:
10.1002/bmc.4143
[Indexed for MEDLINE]

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