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J Biomed Opt. 2017 Nov;22(11):1-4. doi: 10.1117/1.JBO.22.11.110503.

High-refractive index of acrylate embedding resin clarifies mouse brain tissue.

Zhou H1,2, Xiong Y1,2, Wang Y1,2, Wang X1,2, Li P1,2, Gang Y1,2, Liu X1,2, Zeng S1,2.

Author information

1
Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Britton, China.
2
Huazhong University of Science and Technology, Ministry of Education Key Laboratory for Biomedical P, China.

Abstract

Biological tissue transparency combined with light-sheet fluorescence microscopy is a useful method for studying the neural structure of biological tissues. The development of light-sheet fluorescence microscopy also promotes progress in biological tissue clearing methods. The current clarifying methods mostly use liquid reagent to denature protein or remove lipids first, to eliminate or reduce the scattering index or refractive index of the biological tissue. However, denaturing protein and removing lipids require complex procedures or an extended time period. Therefore, here we have developed acrylate resin with a high refractive index, which causes clearing of biological tissue directly after polymerization. This method can improve endogenous fluorescence retention by adjusting the pH value of the resin monomer.

KEYWORDS:

embedding resin; endogenous fluorescence; refractive index; tissue clearing

PMID:
29148271
DOI:
10.1117/1.JBO.22.11.110503
[Indexed for MEDLINE]
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