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Food Chem. 2018 Mar 15;243:11-18. doi: 10.1016/j.foodchem.2017.09.098. Epub 2017 Sep 20.

Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of Nε-carboxymethyl lysine in food samples.

Author information

1
Department of Medical Science, University of Guanajuato, 20 de enero 929 Col. Obregón Leon, Guanajuato CP. 37320, Mexico. Electronic address: armando_gomezojeda@hotmail.com.
2
Department of Chemistry, University of Guanajuato, Lascurain de Retana # 6, Centro Guanajuato, Gto., Guanajuato CP. 36000, Mexico. Electronic address: jaosarahi@gmail.com.
3
Department of Chemistry, University of Guanajuato, Lascurain de Retana # 6, Centro Guanajuato, Gto., Guanajuato CP. 36000, Mexico. Electronic address: katarzyn@ugto.mx.
4
Department of Chemistry, University of Guanajuato, Lascurain de Retana # 6, Centro Guanajuato, Gto., Guanajuato CP. 36000, Mexico. Electronic address: kazimier@ugto.mx.
5
Department of Medical Science, University of Guanajuato, 20 de enero 929 Col. Obregón Leon, Guanajuato CP. 37320, Mexico. Electronic address: gloriabs70@hotmail.com.
6
Department of Medical Science, University of Guanajuato, 20 de enero 929 Col. Obregón Leon, Guanajuato CP. 37320, Mexico. Electronic address: claudialc11@gmail.com.
7
University of Chile, Av. Libertador Bernardo O'Higgins 1058, Santiago, Chile. Electronic address: mariapiadelamaza@gmail.com.
8
Department of Medicine, The Icahn School of Medicine at Mount Sinai, New York, NY, USA. Electronic address: jaime.uribarri@mountsinai.org.
9
Food Bioscience Group, Department of Food Analysis and Bioactivity, Institute of Food Science, Research (CIAL), Spanish National Research Council (CSIC), Nicolas Cabrera 9, Madrid 28049, Spain. Electronic address: mdolores.delcastillo@csic.es.
10
Department of Medical Science, University of Guanajuato, 20 de enero 929 Col. Obregón Leon, Guanajuato CP. 37320, Mexico. Electronic address: marugaray_2000@yahoo.com.

Abstract

Nε-carboxymethyl-lysine (CML) is measured in food, but there is a controversy concerning the most convenient yet reliable method(s) for this task. This work compares three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of CML in several food items. The four methods showed the same decreasing order of CML concentration: beef, bacon>chicken > fish>dairy products>grain products>fruits/vegetables. HPLC-ESI-ITMS/MS results highly correlated with those obtained by ELISA performed with monoclonal CML-antibody (β=0.98, p<0.0001) whereas My Bio Source® kit results were not correlated with those provided by Lamider®. Small differences of CML concentrations in food items prepared by different culinary treatment were clearly distinguished by HPLC-ESI-ITMS/MS, but could not always be detected by ELISA. This work demonstrates a reasonable relationship between CM determined by ELISA and HPLC-ESI-ITMS/MS and therefore supports the implementation of ELISA in food CML/AGEs screening.

KEYWORDS:

ELISA; Food; Liquid chromatography – mass spectrometry; N(ε)-carboxymethyl lysine

PMID:
29146316
DOI:
10.1016/j.foodchem.2017.09.098
[Indexed for MEDLINE]

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