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Toxicol Pathol. 2018 Jan;46(1):47-61. doi: 10.1177/0192623317737254. Epub 2017 Nov 16.

Analytical High-resolution Electron Microscopy Reveals Organ-specific Nanoceria Bioprocessing.

Author information

1
1 Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky Academic Medical Center, Lexington, Kentucky, USA.
2
2 National Institute of Occupational Safety and Health, Cincinnati, Ohio, USA.
3
3 U.S. Air Force Research Laboratory, Dayton, Ohio, USA.
4
4 Department of Anatomical Sciences and Neurobiology, College of Medicine, University of Louisville, Louisville, Kentucky, USA.

Abstract

This is the first utilization of advanced analytical electron microscopy methods, including high-resolution transmission electron microscopy, high-angle annular dark field scanning transmission electron microscopy, electron energy loss spectroscopy, and energy-dispersive X-ray spectroscopy mapping to characterize the organ-specific bioprocessing of a relatively inert nanomaterial (nanoceria). Liver and spleen samples from rats given a single intravenous infusion of nanoceria were obtained after prolonged (90 days) in vivo exposure. These advanced analytical electron microscopy methods were applied to elucidate the organ-specific cellular and subcellular fate of nanoceria after its uptake. Nanoceria is bioprocessed differently in the spleen than in the liver.

KEYWORDS:

bioprocessing; ions; liver; nanoceria; nanoparticles; spleen; transformation

PMID:
29145781
PMCID:
PMC5954437
[Available on 2019-01-01]
DOI:
10.1177/0192623317737254

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