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Diabetologia. 2018 Mar;61(3):526-538. doi: 10.1007/s00125-017-4495-9. Epub 2017 Nov 16.

Considerations and guidelines for mouse metabolic phenotyping in diabetes research.

Author information

1
Montreal Diabetes Research Center and Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), 900 rue Saint-Denis, Office R08-418, Montreal, QC, H2X 0A9, Canada. thierry.alquier@umontreal.ca.
2
Department of Medicine, Université de Montréal, Montreal, QC, Canada. thierry.alquier@umontreal.ca.
3
Montreal Diabetes Research Center and Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), 900 rue Saint-Denis, Office R08-418, Montreal, QC, H2X 0A9, Canada.
4
Department of Medicine, Université de Montréal, Montreal, QC, Canada.

Abstract

Mice are the most commonly used species in preclinical research on the pathophysiology of metabolic diseases. Although they are extremely useful for identifying pathways, mechanisms and genes regulating glucose and energy homeostasis, the specificities of the various mouse models and methodologies used to investigate a metabolic phenotype can have a profound impact on experimental results and their interpretation. This review aims to: (1) describe the most commonly used experimental tests to assess glucose and energy homeostasis in mice; (2) provide some guidelines regarding the design, analysis and interpretation of these tests, as well as for studies using genetic models; and (3) identify important caveats and confounding factors that must be taken into account in the interpretation of findings.

KEYWORDS:

Clamp; Energy balance; Genetic background; Glucose tolerance; Metabolic phenotyping; Mouse; Review

PMID:
29143855
PMCID:
PMC5805661
[Available on 2019-03-01]
DOI:
10.1007/s00125-017-4495-9

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