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Microb Cell Fact. 2017 Nov 15;16(1):203. doi: 10.1186/s12934-017-0813-7.

Regulation of antimicrobial activity and xenocoumacins biosynthesis by pH in Xenorhabdus nematophila.

Author information

1
Research and Development Center of Biorational Pesticides, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, China.
2
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
3
State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
4
School of Agriculture and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.
5
Research and Development Center of Biorational Pesticides, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, China. yhwang@nwsuaf.edu.cn.
6
Shaanxi Research Center of Biopesticide Engineering and Technology, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, China. yhwang@nwsuaf.edu.cn.
7
Shaanxi Research Center of Biopesticide Engineering and Technology, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, China.

Abstract

BACKGROUND:

Xenocoumacin 1 (Xcn1) and Xenocoumacin 2 (Xcn2) are the main antimicrobial compounds produced by Xenorhabdus nematophila. Culture conditions, including pH, had remarkably distinct effects on the antimicrobial activity of X. nematophila. However, the regulatory mechanism of pH on the antimicrobial activity and antibiotic production of this bacterium is still lacking.

RESULTS:

With the increase of initial pH, the antimicrobial activity of X. nematophila YL001 was improved. The levels of Xcn1 and nematophin at pH 8.5 were significantly (P < 0.05) higher than that at pH 5.5 and 7.0. In addition, the expression of xcnA-L, which are responsible for the production of Xcn1 was increased and the expression of xcnMN, which are required for the conversion of Xcn1 to Xcn2 was reduced at pH 8.5. Also, the expression of ompR and cpxR were decreased at pH 8.5.

CONCLUSION:

The alkaline pH environment was found to be beneficial for the production of Xcn1 and nematophin, which in turn led to high antimicrobial activity of X. nematophila at pH 8.5.

KEYWORDS:

Antibiotic production; Antimicrobial activity; Biological control; Xenorhabdus nematophila YL001; pH

PMID:
29141647
PMCID:
PMC5688692
DOI:
10.1186/s12934-017-0813-7
[Indexed for MEDLINE]
Free PMC Article

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