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Tissue Eng Part C Methods. 2018 Mar;24(3):135-145. doi: 10.1089/ten.TEC.2017.0339. Epub 2018 Jan 9.

Sandwiched White Adipose Tissue: A Microphysiological System of Primary Human Adipose Tissue.

Author information

1
1 LSUHSC SOM's Department of Surgery, Louisiana State University Health Sciences Center School of Medicine , New Orleans, Louisiana.
2
2 New Orleans BioInnovation Center , LaCell LLC, New Orleans Louisiana.
3
3 Center for Stem Cell Research and Regenerative Medicine, Tulane University , New Orleans, Louisiana.

Abstract

White adipose tissue (WAT) is a critical organ in both health and disease. However, physiologically faithful tissue culture models of primary human WAT remain limited, at best. In this study we describe a novel WAT culture system in which primary human WAT is sandwiched between tissue-engineered sheets of adipose-derived stromal cells. This construct, called "sandwiched white adipose tissue" (SWAT), can be defined as a microphysiological system (MPS) since it is a tissue-engineered, multicellular, three-dimensional organ construct produced using human cells. We validated SWAT against the National Institutes of Health MPS standards and found that SWAT is viable in culture for 8 weeks, retains physiologic responses to exogenous signaling, secretes adipokines, and engrafts into animal models. These attributes position SWAT as a powerful tool for the study of WAT physiology, pathophysiology, personalized medicine, and pharmaceutical development.

KEYWORDS:

SWAT; fat culture; microphysiological system; tissue engineering; white adipose tissue

PMID:
29141507
DOI:
10.1089/ten.TEC.2017.0339
[Indexed for MEDLINE]

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