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Curr Protoc Stem Cell Biol. 2017 Nov 15;43:2B.8.1-2B.8.17. doi: 10.1002/cpsc.39.

Visualization and Modeling of the In Vivo Distribution of Mesenchymal Stem Cells.

Author information

1
Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, Australia.
2
Department of Biliary-Pancreatic Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
3
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, Australia.
4
School of Medicine, The University of Queensland, Gallipoli Medical Research Foundation, Greenslopes Private Hospital, Greenslopes, Australia.
5
School of Pharmacy and Medical Science, University of South Australia, Adelaide, Australia.

Abstract

This unit describes a protocol for elucidating the in vivo disposition of administered mesenchymal stem cells (MSCs). Specifically, direct visualization of donor cell spatiotemporal distribution and assessment of donor cell quantity in recipient organs are described. Protocols for data analysis are suggested, with the goal of developing a model to characterize and predict the physiological kinetics of administered MSCs. The use of this model is described, suggesting that it can be applied to abnormal conditions and has potential interspecies and inter-route predictive capability. These universal methods can be employed, regardless of the type of stem cell or disease, to guide future experiments and design treatment protocols.

KEYWORDS:

Mesenchymal stem cells; distribution; in vivo imaging; modeling

PMID:
29140565
DOI:
10.1002/cpsc.39
[Indexed for MEDLINE]

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