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Methods Mol Biol. 2018;1788:185-192. doi: 10.1007/7651_2017_88.

Combination Strategy of Quantitative Proteomics Uncovers the Related Proteins of Colorectal Cancer in the Interstitial Fluid of Colonic Tissue from the AOM-DSS Mouse Model.

Author information

1
BGI-SHEHZHEN, Yantian District, Shenzhen, Guangdong, China.
2
Beijing Institute of Genomics, Chinese Academy of Sciences, Chaoyang District, Beijing, China.
3
BGI-Shenzhen, Yantian District, Shenzhen, Guangdong, China. siqiliu@genomics.cn.

Abstract

Quantitative proteome analysis using iTRAQ is an important technique to find disease-related proteins. As an important component of tumor microenvironment, tissue interstitial fluid (TIF) has drawn a great attention for its potential as a source for exploration of the solid tumor biomarkers. On the basis of a mouse model of colorectal cancer (CRC) that was induced by the carcinogenetic reagents, we adopted a quantitative proteome analysis with iTRAQ to discover the CRC-related proteins in the TIFs and with MRM to evaluate the corresponding abundance changes in the individual mouse TIF and serum samples.

KEYWORDS:

AOM-DSS mouse model; Colorectal cancer; MRM; Serum biomarker; Tissue interstitial fluid; iTRAQ

PMID:
29139078
DOI:
10.1007/7651_2017_88
[Indexed for MEDLINE]

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