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Mol Med Rep. 2018 Jan;17(1):1428-1436. doi: 10.3892/mmr.2017.8035. Epub 2017 Nov 13.

Fucoidan inhibits Ca2+ responses induced by a wide spectrum of agonists for G‑protein‑coupled receptors.

Author information

1
Laboratory of Cell Imaging, Henan University of Chinese Medicine, Zhengzhou, Henan 450002, P.R. China.
2
The Research Institute of The McGill University Health Centre, Montreal, QC H4A 3J1, Canada.
3
Photon Medical Research Center, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431‑3192, Japan.

Abstract

Fucoidan, a sulfated polysaccharide extracted from brown seaweed, has been used in traditional Chinese herbal medicine to treat thyroid tumors for many years. Although a number of its cellular effects have been investigated, the role of fucoidan in molecular signaling, particularly in Ca2+ signaling, remains largely unknown. In the present study, the effects of fucoidan on Ca2+ responses in HeLa cells, human umbilical vein endothelial cells and astrocytes were investigated using a wide range of receptor agonists. Fucoidan inhibited the increase in intracellular free calcium concentration that was induced by histamine, ATP, compound 48/80 and acetylcholine. The responses induced by the same agonists in the absence of extracellular Ca2+ were also markedly suppressed by fucoidan. Reverse transcription‑polymerase chain reaction demonstrated that 0.5 and 1.0 mg/ml fucoidan treatment for 3 h decreased histamine receptor 1 expression in HeLa cells. Similarly, the expressions of purinergic receptor P2Y, G‑protein coupled (P2YR)1, P2YR2 and P2YR11 were significantly downregulated within cells pretreated with 1.0 mg/ml fucoidan for 3 h, and 0.5 mg/ml fucoidan significantly inhibited P2YR1 and P2YR11 expression. The results demonstrated that fucoidan may exert a wide spectrum of inhibitory effects on Ca2+ responses and that fucoidan may inhibit a number of different G‑protein coupled receptors associated with Ca2+ dynamics.

PMID:
29138822
PMCID:
PMC5780082
DOI:
10.3892/mmr.2017.8035
[Indexed for MEDLINE]
Free PMC Article

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