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Sci Rep. 2017 Nov 14;7(1):15577. doi: 10.1038/s41598-017-15781-z.

Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development.

Author information

1
International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India.
2
School of Life Sciences, University of Nottingham, Nottingham, NG72UH, UK.
3
Institute of Cell Biology, University of Bern, Bern, 3012, Switzerland.
4
Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK.
5
Francis Crick Institute, London, NW1 1AT, UK.
6
Protein and Nucleic Acid Chemistry Laboratory, University of Leicester, Leicester, LE2 7LX, UK.
7
Department of Cancer studies, University of Leicester, Leicester, LE2 7LX, UK.
8
International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India. pawanm@icgeb.res.in.
9
School of Life Sciences, University of Nottingham, Nottingham, NG72UH, UK. rita.tewari@nottingham.ac.uk.

Abstract

Plasmodium parasites, the causative agents of malaria, possess a distinctive membranous structure of flattened alveolar vesicles supported by a proteinaceous network, and referred to as the inner membrane complex (IMC). The IMC has a role in actomyosin-mediated motility and host cell invasion. Here, we examine the location, protein interactome and function of PhIL1, an IMC-associated protein on the motile and invasive stages of both human and rodent parasites. We show that PhIL1 is located in the IMC in all three invasive (merozoite, ookinete-, and sporozoite) stages of development, as well as in the male gametocyte and locates both at the apical and basal ends of ookinete and sporozoite stages. Proteins interacting with PhIL1 were identified, showing that PhIL1 was bound to only some proteins present in the glideosome motor complex (GAP50, GAPM1-3) of both P. falciparum and P. berghei. Analysis of PhIL1 function using gene targeting approaches indicated that the protein is required for both asexual and sexual stages of development. In conclusion, we show that PhIL1 is required for development of all zoite stages of Plasmodium and it is part of a novel protein complex with an overall composition overlapping with but different to that of the glideosome.

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