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Oncol Res. 2018 Mar 5;26(2):315-322. doi: 10.3727/096504017X15067856789781. Epub 2017 Oct 3.

MicroRNA-433 Represses Proliferation and Invasion of Colon Cancer Cells by Targeting Homeobox A1.

Author information

1
Emergency Department, 5th Hospital of Dongguan City, Dongguan, P.R. China.
2
Hepatobiliary Surgery Department, 5th Hospital of Dongguan City, Dongguan, P.R. China.
3
Cardiovascular Department, TungWah Hospital of Sun-Yat Sen University, Donggguan, P.R. China.

Abstract

The aberrant expression of miR-433 has been validated in some types of cancers. However, the expression profile and the biological function of miR-433 on colon cancer are still elusive. This study was designed to investigate the function of miR-433 on the proliferation and invasion of colon cancer cells. We detected the expression of miR-433 in colon cancer tissues, adjacent normal tissues, and cell lines. CCK8 and Transwell assays were performed to explore the impact of miR-433 on colon cancer cell proliferation and invasion. The luciferase reporter assay was applied to identify the direct target of miR-433. The results demonstrated that miR-433 was downregulated in colon cancer tissues and cell lines when compared with the control. Overexpression of miR-433 significantly suppressed the ability of colon cancer cell proliferation and invasion, whereas knockdown of miR-433 remarkably enhanced cell proliferation and invasion. Homeobox A1 (HOXA1) was identified as a target of miR-433, and it mediated the functions of miR-433 on colon cancer cells. To conclude, we revealed that miR-433 was downregulated in colon cancer, and it inhibited colon cancer cell proliferation and invasion by directly targeting HOXA1.

[Indexed for MEDLINE]

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