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Talanta. 2018 Feb 1;178:1067-1076. doi: 10.1016/j.talanta.2017.07.079. Epub 2017 Aug 3.

Unparalleled sample treatment throughput for proteomics workflows relying on ultrasonic energy.

Author information

1
BIOSCOPE Group, UCIBIO-REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, Caparica 2829-516, Portugal; ProteoMass Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal.
2
Mio and pain lab, CEDOC, NOVA medical School, Universidade NOVA de Lisboa, Portugal; CHLO, Egas Moniz Hospital, Rheumatology Department, Portugal.
3
BIOSCOPE Group, UCIBIO-REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, Caparica 2829-516, Portugal; ProteoMass Scientific Society, Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal. Electronic address: jlcm@fct.unl.pt.

Abstract

We report on the new microplate horn ultrasonic device as a powerful tool to speed proteomics workflows with unparalleled throughput. 96 complex proteomes were digested at the same time in 4min. Variables such as ultrasonication time, ultrasonication amplitude, and protein to enzyme ratio were optimized. The "classic" method relying on overnight protein digestion (12h) and the sonoreactor-based method were also employed for comparative purposes. We found the protein digestion efficiency homogeneously distributed in the entire microplate horn surface using the following conditions: 4min sonication time and 25% amplitude. Using this approach, patients with lymphoma and myeloma were classified using principal component analysis and a 2D gel-mass spectrometry based approach. Furthermore, we demonstrate the excellent performance by using MALDI-mass spectrometry based profiling as a fast way to classify patients with rheumatoid arthritis, systemic lupus erythematosus, and ankylosing spondylitis. Finally, the speed and simplicity of this method were demonstrated by clustering 90 patients with knee osteoarthritis disease (30), with a prosthesis (30, control group) and healthy individuals (30) with no history of joint disease. Overall, the new approach allows profiling a disease in just one week while allows to match the minimalism rules as outlined by Halls.

KEYWORDS:

Biomarker; Cancer; Disease; Method; Sample treatment; Ultrasound

PMID:
29136797
DOI:
10.1016/j.talanta.2017.07.079
[Indexed for MEDLINE]

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