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Arch Virol. 2018 Feb;163(2):545-548. doi: 10.1007/s00705-017-3618-4. Epub 2017 Nov 14.

Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method.

Author information

1
Department of Clinical Medicine, XuZhou Medical University, Xuzhou, People's Republic of China.
2
School of Life science, Taizhou University, Taizhou, People's Republic of China.
3
Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
4
College of Life and Environment Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
5
Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
6
Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China. cym59059@163.com.
7
Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China. kzhao118@163.com.
8
Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China. kzhao118@163.com.

Abstract

Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.

KEYWORDS:

LAMP; Potyvirus; ornamental plant; virus detection

PMID:
29134340
DOI:
10.1007/s00705-017-3618-4
[Indexed for MEDLINE]

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