Format

Send to

Choose Destination
Mol Cancer Ther. 2018 Jan;17(1):290-296. doi: 10.1158/1535-7163.MCT-17-0436. Epub 2017 Nov 13.

Clinical Application of Circulating Tumor DNA in the Genetic Analysis of Patients with Advanced GIST.

Author information

1
Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road, Nanjing, Jiangsu Province, China.
2
Nanjing Geneseeq Biotechnology Inc., Nanjing, Jiangsu Province.
3
Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
4
Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province.
5
Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road, Nanjing, Jiangsu Province, China. xuzekuan@njmu.edu.cn.
#
Contributed equally

Abstract

Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumor of digestive tract. In the past, tissue biopsy was the main method for the diagnosis of GISTs. Although, circulating tumor DNA (ctDNA) detection by next-generation sequencing (NGS) may be a feasible and replaceable method for diagnosis of GISTs. We retrospectively analyzed the data for ctDNA and tissue DNA detection from 32 advanced GIST patients. We found that NGS obviously increased the positive rate of ctDNA detection. ctDNA detection identified rare mutations that were not detected in tissue DNA detection. Tumor size and Ki-67 were significant influencing factors of the positive rate of ctDNA detection and concordance between ctDNA and tissue DNA detection. In all patients, the concordance rate between ctDNA and tissue DNA detection was 71.9%, with moderate concordance, but the concordance was strong for patients with tumor size > 10 cm or Ki-67 > 5%. Tumor size, mitotic figure, Ki-67, and ctDNA mutation type were the significant influencing factors of prognosis, but only tumor size and ctDNA mutation type, were the independent prognostic factors for advanced GIST patients. We confirmed that ctDNA detection by NGS is a feasible and promising method for the diagnosis and prognosis of advanced GIST patients. Mol Cancer Ther; 17(1); 290-6. ©2017 AACR.

PMID:
29133619
DOI:
10.1158/1535-7163.MCT-17-0436
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center