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J Exp Clin Cancer Res. 2017 Nov 13;36(1):158. doi: 10.1186/s13046-017-0630-1.

MiR-361-5p inhibits glycolytic metabolism, proliferation and invasion of breast cancer by targeting FGFR1 and MMP-1.

Author information

1
Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Street, Nangang District, Harbin, China.
2
Department of Ultrasound, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.
3
Computer Center, the Fifth Hospital of Harbin, Harbin, China.
4
Department of Endoscopy, Harbin Medical University Cancer Hospital, Harbin, China.
5
Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Street, Nangang District, Harbin, China. baoliangguo2013@163.com.

Abstract

BACKGROUND:

MicroRNAs function as key regulators in various human cancers, including breast cancer (BC). MiR-361-5p has been proved to be a tumor suppressor in colorectal cancer and gastric cancer in our previous study. In this study, we aim to find out the function of miR-361-5p in breast cancer progression and elaborate the mechanism that miR-361-5p acts its function in breast cancer.

METHODS AND RESULTS:

Here we reported that miR-361-5p was down-regulated in breast cancer tissue compared with normal breast tissue and the expression of miR-361-5p was positively associated with prognosis in BC patients. Functional studies showed that overexpression of miR-361-5p suppressed the proliferation, invasion and metastasis of breast cancer cells both in vivo and in vitro. Mechanistically, we found that miR-361-5p inhibited the proliferation of BC cells by suppressing glycolysis. FGFR1, a promoter of glycolysis-related enzyme, was identified as the target of miR-361-5p that promoted glycolysis and repressed oxidative phosphorylation. Furthermore, we demonstrated that miR-361-5p inhibited breast cancer cells invasion and metastasis by targeting MMP-1. An inverse expression pattern was also found between miR-361-5p and FGFR1 or MMP-1 in a cohort of 60 BC tissues.

CONCLUSION:

Our results indicate that miR-361-5p inhibits breast cancer cells glycolysis and invasion by respectively repressing FGFR1 and MMP-1, suggesting that miR-361-5p and its targets may serve as therapeutic targets in breast cancer treatment.

KEYWORDS:

FGFR1; Glycolysis; MMP-1; miR-361-5p

PMID:
29132384
PMCID:
PMC5683540
DOI:
10.1186/s13046-017-0630-1
[Indexed for MEDLINE]
Free PMC Article

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