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BMC Vet Res. 2017 Nov 13;13(1):333. doi: 10.1186/s12917-017-1251-9.

Frequency of canine nt230(del4) MDR1 mutation in prone pure breeds, their crosses and mongrels in Israel - insights from a worldwide comparative perspective.

Author information

1
Shamir Research Institute, University of Haifa, P.O.Box 97, 1290000, Kazerin, Israel. Yarond@gri.org.il.
2
Zefat Academic College, Zefat, Israel. Yarond@gri.org.il.
3
GGA - Galil Genetic Analysis Ltd, P.O.Box 3664, 12900, Kazerin, Israel. Yarond@gri.org.il.
4
Specialist in the Fields of Genetics, Epidemiology and Molecular Biology, Rehovot, Israel.
5
GGA - Galil Genetic Analysis Ltd, P.O.Box 3664, 12900, Kazerin, Israel.
6
Shamir Research Institute, University of Haifa, P.O.Box 97, 1290000, Kazerin, Israel.
7
Tel Hai College, 12210, Tel-Hai, Israel.
8
Vetmarket Ltd. Industrial Park Hevel Modiin, P.O.Box 960, 6085001, Shoham, Israel.
9
Zefat Academic College, Zefat, Israel.
10
Faculty of Medicine in the Galilee, Bar-Ilan University & Baruch Padeh Medical Center, Poria, Israel.

Abstract

BACKGROUND:

Sensitivity to macrocyclic lactones, which are commonly used in veterinary clinics, was first found in Rough Collies, and was attributed in 2001 to a 4 bp deletion in the MDR1 gene. The list of affected breeds currently includes 13 breeds. Researchers from different countries and continents examined the allelic frequencies of the nt230(del4) MDR1 mutation, emphasizing the clinical importance of this test not only to mutation-prone dogs, but also to their crosses and mongrels, since treatment of a deletion carrier with these compounds may lead to its death. In this study, the allelic frequencies of nt230(del4) MDR1 mutation in affected breeds, their crosses, unrelated pure breeds and mongrels are reported for the state of Israel (n = 1416 dogs). The Israeli data were compared with reports from the US, Europe, UK, Australia and Japan.

RESULTS:

The allelic frequencies of nt230(del4) MDR1 mutation in Israel for Australian, Swiss and German Shepherds (31%, 17% and 2.4%, respectively) are similar to the corresponding frequencies worldwide, much higher for Border Collies (4.8%), twice lower for Rough Collies (28%, compared to 55% or more elsewhere), and ~1% for mongrels. The frequencies for crosses of Australian Shepherd and Border Collies in Israel are 4 and 1.6 times lower, respectively, compared to the frequencies for the respective pure breeds.

CONCLUSIONS:

This work, that for the first time presents the frequency of nt230(del4) MDR1 mutation in Israel, along with a worldwide survey, has implications for clinicians, owners and breeders of sheepdogs and their crosses and supports the need for extra care in treatment and in future breeding. Of note, the relative proportion of affected breeds, in the overall tested dogs, might be higher than their actual proportion in Israel due to directed samples collection by veterinarians for clinical purposes, as these are mainly limited to certain affected breeds or dogs that resemble them.

KEYWORDS:

Drug sensitivity; Macrocyclic lactones; Sheepdogs; Taqman assay; nt230(del) MDR1 mutation

PMID:
29132368
PMCID:
PMC5683241
DOI:
10.1186/s12917-017-1251-9
[Indexed for MEDLINE]
Free PMC Article

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