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Methods Mol Biol. 2018;1673:363-370. doi: 10.1007/978-1-4939-7309-5_27.

Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of õ-Hemolysin with High Performance Liquid Chromatography.

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Department of Dermatology, Emory University School of Medicine, 615 Michael St., Whitehead 105L, Atlanta, GA, 30322, USA.
Center for the Study of Human Health, Emory University College of Arts and Sciences, 550 Asbury Circle, Candler Library 107, Atlanta, GA, 30322, USA.
Department of Immunology and Microbiology, The University of Colorado School of Medicine, Mail Stop 8333, Research Complex 1 North, 12800 E. 19th Ave., Rm. P18-9101, Aurora, CO, 80045, USA.


Quorum sensing plays a major role in regulation of virulence factor production by staphylococci. Chemical inhibitors that block this process and prevent the production of exotoxins and exoenzymes could have medical utility for infection prophylaxis and therapy. Here, we describe a high performance liquid chromatography method amenable to medium throughput screening for staphylococcal quorum sensing inhibitors by quantification of õ-hemolysin, a direct protein output of this system.


Accessory gene regulator; High performance liquid chromatography; Staphylococcal quorum sensing inhibitors; Staphylococcus aureus; õ-Toxin

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