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Methods Mol Biol. 2018;1673:117-128. doi: 10.1007/978-1-4939-7309-5_10.

Detection and Quantification of Butyrolactones from Streptomyces.

Author information

1
Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, School of Chemistry, Faculty of Science and Engineering, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
2
Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, School of Chemistry, Faculty of Science and Engineering, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK. eriko.takano@manchester.ac.uk.

Abstract

In Streptomyces, the onset of antibiotic production and sporulation is coordinated through small diffusible molecules known as γ-butyrolactones (GBLs). These are active in very low amounts, and their extraction and characterization are challenging. Here we describe a rapid, small-scale method for the extraction of GBL from Streptomyces coelicolor, from both solid and liquid cultures, which provides sufficient material for subsequent bioassays and partial characterization. We also present two different bioassay techniques for the detection and quantification of the GBL content in the extracts: the antibiotic bioassay and the kanamycin bioassay.

KEYWORDS:

Antibiotic bioassay; Antibiotic production regulation; Gamma-butyrolactones; Kanamycin bioassay; Small-scale gamma-butyrolactone extraction

PMID:
29130169
DOI:
10.1007/978-1-4939-7309-5_10
[Indexed for MEDLINE]

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