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J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Nov 15;1068-1069:358-364. doi: 10.1016/j.jchromb.2017.11.003. Epub 2017 Nov 4.

Fractionation separation of human plasma proteins using HPLC with a homemade iron porphyrin based monolithic column.

Author information

1
College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, 071002, China.
2
North China Pharmaceutical Corporation New Drug Research & Development Company Ltd., Shijiazhuang, 050015, China.
3
College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, 071002, China. Electronic address: bailigai@163.com.
4
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, 071002, China.

Abstract

In this work a polymer monolithic column was fabricated within the confines of a stainless steel column (50×4.6mm i.d.) via radical polymerization by using iron porphyrin and butyl methacrylate as co-monomers, ethylene glycol dimethacrylate as crosslinking agent, ethylene glycol, isopropyl alcohol and N, N-dimethylformamide as tri-porogens, benzoyl peroxide and N,N-dimethylaniline as initiators. The resulting monolithic column was characterized by elemental analysis, scanning electron microscopy, nitrogen adsorption BET surface area, and mercury intrusion porosimetry, respectively. Results showed that the homemade monolith occupied relatively uniform pore structure, low back pressure, and enhanced selectivity for proteins in complex bio-samples. The present work described a simple and efficient method for "fractionation separation" of human plasma proteins, and it is a promising separation method for complex bio-samples in proteomic research.

KEYWORDS:

Bio-sample; Fractionation separation; High performance liquid chromatography; Human plasma; Iron porphyrin; Polymer monolithic column

PMID:
29127055
DOI:
10.1016/j.jchromb.2017.11.003
[Indexed for MEDLINE]

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