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J Craniomaxillofac Surg. 2017 Dec;45(12):2084-2091. doi: 10.1016/j.jcms.2017.10.005. Epub 2017 Oct 14.

BMP-2 plasmid DNA-loaded chitosan films - A new strategy for bone engineering.

Author information

1
Department of Stomatology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Qin Chun Road 79, 310003 Hangzhou, China.
2
Department of Stomatology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Qin Chun Road 79, 310003 Hangzhou, China. Electronic address: linjun2@zju.edu.cn.
3
Department of Polymer Science and Engineering, Zhejiang University, Zhe Da Road 38, 310027 Hangzhou, China.
4
Department of Plastic Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Qin Chun Road 79, 310003 Hangzhou, China.
5
Department of Prosthodontics, Propaedeutics and Dental Materials, Christian-Albrechts University at Kiel, Arnold-Heller-Straße 16, 24105 Kiel, Germany. Electronic address: cmehl@proth.uni-kiel.de.

Abstract

OBJECTIVES:

Bone defects are common in every area of medicine and remain a clinical challenge. Tissue engineering has led to promising new strategies in accelerating bone repair. Bone morphogenetic proteins (BMPs) play crucial roles in bone regeneration, but are required in supra-physiological doses, which are expensive and produce severe side effects.

METHODS:

To address these issues, we prepared BMP-2 plasmid DNA-loaded chitosan films, and examined their effects on mouse osteoblast-like MC3T3-E1 cell morphology, proliferation, and runt-related transcription factor 2 (RUNX2) expression. In vivo testing was performed using calvarial critical-sized defects and histomorphometry in 36 Sprague-Dawley rats. Unloaded chitosan films and empty defects served as controls.

RESULTS:

In contrast to the controls, cells grown on BMP-2 plasmid DNA-loaded chitosan films had well established filopodia and lamellipodia, significantly higher proliferation 2, 4, and 6 days post-seeding (P ≤ 0.05), and higher nuclear RUNX2 expression. In vivo, new bone growth was significantly greater in the BMP-2 group than in the control groups at 4, 8, and 12 weeks (P ≤ 0.01).

CONCLUSIONS:

Based on our study findings, BMP-2 plasmid DNA-loaded chitosan films provide an effective strategy for GBR, combining cellular compatibility with biocapability in vivo.

KEYWORDS:

Bone; Bone morphogenetic protein 2; Chitosan; DNA; Drug delivery systems; Histology

PMID:
29126771
DOI:
10.1016/j.jcms.2017.10.005
[Indexed for MEDLINE]

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