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Leg Med (Tokyo). 2018 Jan;30:1-4. doi: 10.1016/j.legalmed.2017.10.013. Epub 2017 Oct 31.

A capillary electrophoresis method for identifying forensically relevant body fluids using miRNAs.

Author information

1
Department of Forensic Science, College of Criminal Justice, Sam Houston State University, 1003 Bowers Blvd., Huntsville, TX 77340-2525, United States. Electronic address: cam115@shsu.edu.
2
Department of Forensic Science, Virginia Commonwealth University, Box 843079, Richmond, VA 23284, United States.
3
Department of Forensic Science, College of Criminal Justice, Sam Houston State University, 1003 Bowers Blvd., Huntsville, TX 77340-2525, United States.

Abstract

Body fluid identification (BFID) can provide crucial information during the course of an investigation. In recent years, microRNAs (miRNAs) have shown considerable body fluid specificity, are able to be co-extracted with DNA, and their small size (18-25 nucleotides) make them ideal for analyzing highly degraded forensic samples. In this study, we designed a preliminary 8-marker system for BFID including an endogenous reference gene (let-7g) to differentiate between venous blood (miR-451a and miR-142-3p), menstrual blood (miR-141-3p and miR-412-3p), semen (miR-891a and miR-10b), and saliva (miR-205) using a capillary electrophoresis approach. This panel uses a linear primer system in order to incorporate additional miRNA markers by forming a multiplex system. The miRNA system was able to distinguish between venous blood, menstrual blood, semen, and saliva using a rudimentary data interpretation strategy. All STR amplifications from co-extracted DNA yielded complete profiles from human identification purposes.

KEYWORDS:

Body fluid identification; Capillary electrophoresis; Forensic genetics; MicroRNAs

PMID:
29125963
DOI:
10.1016/j.legalmed.2017.10.013
[Indexed for MEDLINE]

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