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Angew Chem Int Ed Engl. 2017 Dec 18;56(51):16297-16301. doi: 10.1002/anie.201710269. Epub 2017 Nov 22.

An in situ Dynamic Continuum of Supramolecular Phosphoglycopeptides Enables Formation of 3D Cell Spheroids.

Author information

1
Department of chemistry, Brandeis University, 415 South St, Waltham, MA, 02454, USA.
2
Department of Biology, Brandeis University, 415 South St, Waltham, MA, 02454, USA.

Abstract

Higher-order assemblies of proteins, with a structural and dynamic continuum, is an important concept in biology, but these insights have yet to be applied in designing biomaterials. Dynamic assemblies of supramolecular phosphoglycopeptides (sPGPs) transform a 2D cell sheet into 3D cell spheroids. A ligand-receptor interaction between a glycopeptide and a phosphopeptide produces sPGPs that form nanoparticles, which transform into nanofibrils upon partial enzymatic dephosphorylation. The assemblies form dynamically and hierarchically in situ on the cell surface, and interact with the extracellular matrix molecules and effectively abolish contact inhibition of locomotion (CIL) of the cells. Integrating molecular recognition, catalysis, and assembly, these active assemblies act as a dynamic continuum to disrupt CIL, thus illustrating a new kind of biomaterial for regulating cell behavior.

KEYWORDS:

cell spheroids; enzymes; extracellular matrix; self-assembly; vancomycin

PMID:
29125896
PMCID:
PMC5857944
DOI:
10.1002/anie.201710269
[Indexed for MEDLINE]
Free PMC Article

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