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Nat Commun. 2017 Nov 9;8(1):1396. doi: 10.1038/s41467-017-01557-6.

Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles.

Author information

1
Centro Nacional de Biotecnologia, CNB-CSIC, Department of Macromolecular Structures, Cell Structure Lab, 28049, Madrid, Spain.
2
Département de Virologie, Institut Pasteur, Unité de Génétique Moléculaire des Virus à ARN, 75015, Paris, France.
3
CNRS, UMR3569, 75015, Paris, France.
4
Université Paris Diderot, Sorbonne Paris Cité, EA302, 75015, Paris, France.
5
Center for Innovation and Technological Research, Ultrastructural Bio-imaging, Institut Pasteur, 75015, Paris, France.
6
Département de Biologie Cellulaire et Infection, Institut Pasteur, Unité des Interactions Bactéries-Cellules, 75015, Paris, France.
7
INSERM, U220 8. INRA, USC 2020, 75015, Paris, France.
8
Unité de Recherche Yersinia, Institut Pasteur, 75015, Paris, France.
9
Centre National de Référence 'Peste et autres Yersinioses', 75015, Paris, France.
10
Centre Collaborateur OMS de Référence et Recherche 'Yersinioses', Institut Pasteur, Unité des Interactions Bactéries-Cellules, 75015, Paris, France.
11
Centro Nacional de Biotecnologia, CNB-CSIC, Department of Macromolecular Structures, Cell Structure Lab, 28049, Madrid, Spain. crisco@cnb.csic.es.
12
Département de Virologie, Institut Pasteur, Unité de Génétique Moléculaire des Virus à ARN, 75015, Paris, France. nadia.naffakh@pasteur.fr.
13
CNRS, UMR3569, 75015, Paris, France. nadia.naffakh@pasteur.fr.
14
Université Paris Diderot, Sorbonne Paris Cité, EA302, 75015, Paris, France. nadia.naffakh@pasteur.fr.

Abstract

Transport of neo-synthesized influenza A virus (IAV) viral ribonucleoproteins (vRNPs) from the nucleus to the plasma membrane involves Rab 11 but the precise mechanism remains poorly understood. We used metal-tagging and immunolabeling to visualize viral proteins and cellular endomembrane markers by electron microscopy of IAV-infected cells. Unexpectedly, we provide evidence that the vRNP components and the Rab11 protein are present at the membrane of a modified, tubulated endoplasmic reticulum (ER) that extends all throughout the cell, and on irregularly coated vesicles (ICVs). Some ICVs are found very close to the ER and to the plasma membrane. ICV formation is observed only in infected cells and requires an active Rab11 GTPase. Against the currently accepted model in which vRNPs are carried onto Rab11-positive recycling endosomes across the cytoplasm, our findings reveal that the endomembrane organelle that is primarily involved in the transport of vRNPs is the ER.

PMID:
29123131
PMCID:
PMC5680169
DOI:
10.1038/s41467-017-01557-6
[Indexed for MEDLINE]
Free PMC Article

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